A food-grade and senescent cell-targeted fisetin delivery system based on whey protein isolate-galactooligosaccharides Maillard conjugate

Cellular senescence is the results of aging and age-related diseases, development anti-aging methods may improve health extend longevity. The natural flavonol fisetin has been shown to antagonize in vitro increases longevity vivo, but poor water solubility limited bioavailability. In this study, a food-grade senescent cell-targeted delivery system for was developed based on whey protein isolate-galactooligosaccharides (WPI-GOS) Maillard conjugate, which could recognize associated β-galactosidase in cells. nanoparticles possessed high encapsulation efficiency, excellent dispersibility water, good storage stability well biocompatibility. Moreover, they effectively accumulate retain cells with cell-targeting efficacy, inhibit oxidative stress-induced cellular vitro. Thus, novel nanoparticle WPI-GOS conjugate showed promise deliver hydrophobic bioactive ingredients like their bioavailability anti-senescence effect.